All lanes : Anti-FREM2 antibody (ab75803) at 1 µg/mlLane 1 : EBS E14 TG2A Day 3 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell LysateLane 2 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell LysateLane 3 : EBS E14 TG2A Day 8 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab75803 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.