All lanes : Anti-FRS2 antibody [EPR14724] (ab183492) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-FRS2 antibody [EPR14724] (ab183492) at 1/2000 dilution + SH-SY5Y cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling FRS2 with ab183492 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of FRS2 in Human fetal brain lysate immunoprecipitated using ab183492 at 1/50 dilution (Lane 1). Lane 2: TBS instead of Human fetal brain lysate.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.