Performed under reducing conditions.
All lanes : Anti-Fructose 6 Phosphate Kinase antibody [ EPR10734(B)] (ab154804) at 1/1000 dilutionLane 1 : Fetal brain lysateLane 2 : Fetal heart lysateLane 3 : Ramos lysateLane 4 : SHSY5Y lysateLane 5 : HeLa lysateLysates/proteins at 10 µg per lane.
Immunofluorescence analysis of HeLa cells labeling Fructose 6 Phosphate Kinase with ab154804 at 1/50 dilution.
Flow cytometric analysis of permeabilized SHSY5Y cells labeling Fructose 6 Phosphate Kinase with ab154804 at 1/100 dilution (red) or a rabbit IgG (green - negative control).