Anti-FUBP1 antibody [EPR12327] (ab181111) at 1/5000 dilution + Jurkat cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-FUBP1 antibody [EPR12327] (ab181111) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling FUBP1 with ab181111 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling FUBP1 with ab181111 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling FUBP1 with ab181111 at 1/500 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling FUBP1 with ab181111 at 1/90 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.