Immunohistochemical analysis of paraffin embedded Human colon tissue labeling FUBP3 with ab181122 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin embedded Human kidney clear cell carcinoma tissue labeling FUBP3 with ab181122 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling FUBP3 with ab181122 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
Anti-FUBP3 antibody [EPR13174-61] (ab181122) at 1/5000 dilution + HepG2 lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-FUBP3 antibody [EPR13174-61] (ab181122) at 1/20000 dilutionLane 1 : MCF7 lysateLane 2 : U87-MG lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution