All lanes : Anti-Fumarylacetoacetate hydrolase antibody (ab81087) at 1/500 dilutionLane 1 : Whole cell lysate derived from liver extract from FAH wild type mice Lane 2 : Whole cell lysate derived from liver extract from FAH wild type mice Lane 3 : Whole cell lysate derived from FAH-liver extract from z alleles transgenic miceLane 4 : Whole cell lysate derived from FAH-liver extract from z alleles transgenic mice Lane 5 : Whole cell lysate derived from liver extract from siLucFAH mice
ab81087 at 2µg/ml staining Fumarylacetoacetate hydrolase by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded mouse liver sections), followed by HRP linked secondary antibody, and visualized by AEC substrate, and counterstaining by hematoxylin.
ICC/IF image of ab81087 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81087, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.