Anti-FXR1 antibody

• Supplier: Abcam
• Catalog: ab51970
• Prices: $370.00
• Sizes: 200 µl
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF WB ELISA
• Species Reactivities: Mouse, Human, Rat, Dog
• Antigen: Synthetic peptide: RIEGDNENKLPRED , corresponding to amino acids 317/330 of Human FXR1 Run BLAST with Run BLAST with
• Description: Goat Polyclonal
• Gene: FXR1
• Conjugate: Unconjugated

Product images

Anti-FXR1 antibody (ab51970) at 0.2 µg/ml + NIH/3T3 cell lysate in RIPA buffer at 35 µg

ICC/IF image of ab51970 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab51970 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51970, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Deregulation of Fragile X-related protein 1 by the lipodystrophic lamin A p.R482W - Deregulation of Fragile X-related protein 1 by the lipodystrophic lamin A p.R482W

Oldenburg AR, Delbarre E, Thiede B, Vigouroux C, Collas P. Hum Mol Genet. 2014 Mar 1;23(5):1151-62.

FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 - FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9

Xu XL, Zong R, Li Z, Biswas MH, Fang Z, Nelson DL, Gao FB. J Neurosci. 2011 Sep 28;31(39):13705-9.

Fragile X-related protein FXR1 controls post-transcriptional suppression of - Fragile X-related protein FXR1 controls post-transcriptional suppression of

Khera TK, Dick AD, Nicholson LB. FEBS J. 2010 Jul;277(13):2754-65.