Anti-G-protein coupled receptor 30 antibody
| Name | Anti-G-protein coupled receptor 30 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39742 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human GPCR GPR30 |
| Blocking Peptide | Human G-protein coupled receptor 30 peptide |
| Description | Rabbit Polyclonal |
| Gene | GPER1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-G-protein coupled receptor 30 antibody (ab39742) at 1 µg/ml + Brain (Human) Membrane Lysate - adult normal tissue (ab29456) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab39742 staining cultured rat primary astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab39742 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. In red astrocytes can be observed (monoclonal anti GFAP).See Abreview
ICC/IF image of ab39742 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39742 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of Human ovarian endometriosis tissue, staining G-protein coupled receptor 30 with ab39742 at 1/50 dilution.
Product References
The G protein-coupled estrogen receptor (GPER) is expressed in two different - The G protein-coupled estrogen receptor (GPER) is expressed in two different
Samartzis EP, Noske A, Meisel A, Varga Z, Fink D, Imesch P. PLoS One. 2014 Jan 8;9(1):e83296.
The G protein-coupled receptor GPR30 mediates the proliferative and invasive - The G protein-coupled receptor GPR30 mediates the proliferative and invasive
Du GQ, Zhou L, Chen XY, Wan XP, He YY. Biochem Biophys Res Commun. 2012 Apr 6;420(2):343-9. doi:
Estrogen facilitates spinal cord synaptic transmission via membrane-bound - Estrogen facilitates spinal cord synaptic transmission via membrane-bound
Zhang Y, Xiao X, Zhang XM, Zhao ZQ, Zhang YQ. J Biol Chem. 2012 Sep 28;287(40):33268-81. Epub 2012 Aug 6.
Expression of the G protein-coupled estrogen receptor (GPER) in endometriosis: a - Expression of the G protein-coupled estrogen receptor (GPER) in endometriosis: a
Samartzis N, Samartzis EP, Noske A, Fedier A, Dedes KJ, Caduff R, Fink D, Imesch P. Reprod Biol Endocrinol. 2012 Apr 20;10:30.
Estradiol-induced estrogen receptor-alpha trafficking. - Estradiol-induced estrogen receptor-alpha trafficking.
Bondar G, Kuo J, Hamid N, Micevych P. J Neurosci. 2009 Dec 2;29(48):15323-30.