Anti-G3BP antibody
| Name | Anti-G3BP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39533 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF Electron microscopy WB ELISA IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic non-phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 |
| Description | Rabbit Polyclonal |
| Gene | G3BP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39533 diluted 1:50, with (right) or without (left) pre-incubation with synthetic peptide.
All lanes : Anti-G3BP antibody (ab39533) at 1/500 dilutionLane 1 : COLO 205 cell extractLane 2 : COS cell extractLane 3 : blocking peptide control
ICC/IF image of ab39533 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39533, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Fathinajafabadi A, Perez-Jimenez E, Riera M, Knecht E, Gonzalez-Duarte R. PLoS One. 2014 Feb 3;9(2):e87898.
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Fung G, Ng CS, Zhang J, Shi J, Wong J, Piesik P, Han L, Chu F, Jagdeo J, Jan E, Fujita T, Luo H. PLoS One. 2013 Nov 18;8(11):e79546.
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Shim JH, Su ZY, Chae JI, Kim DJ, Zhu F, Ma WY, Bode AM, Yang CS, Dong Z. Cancer Prev Res (Phila). 2010 May;3(5):670-9. doi:
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Sano E, Shono S, Tashiro K, Konishi H, Yamauchi E, Taniguchi H. Biochim Biophys Acta. 2008 Jun;1783(6):1036-47. doi: