Anti-G3BP antibody [EPR13986(B)]

• Supplier: Abcam
• Catalog: ab181150
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13986(B)
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide within Human G3BP aa 400 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: G3BP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-G3BP antibody [EPR13986(B)] (ab181150) at 1/5000 dilutionLane 1 : Jurkat cell lysate with NFDM/TBSTLane 2 : Ramos cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-G3BP antibody [EPR13986(B)] (ab181150) at 1/1000 dilutionLane 1 : Hela cell lysate with NFDM/TBSTLane 2 : 293 cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human colon tissue staining G3BP using ab181150 at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells staining G3BP using ab181150 at 1/100 dilution, and Alexa Fluor®555 stained Goat anti Rabbit IgG at 1/200 dilution as a secondary antibody (red). Dapi counterstain (blue)

Western blot analysis of G3BP in immunoprecipitation pellets from Ramos cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST