Anti-G3BP2 antibody
| Name | Anti-G3BP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab86135 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Dog, Turkey, Pig, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide, corresponding to a region within amino acids 275 - 325 of Human G3BP2 (NP_036429 |
| Description | Rabbit Polyclonal |
| Gene | G3BP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgdeveloped using the ECL technique
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135. 20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 30 seconds.
ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Downregulation of G3BPs inhibits the growth, migration and invasion of human lung - Downregulation of G3BPs inhibits the growth, migration and invasion of human lung
Zhang H, Zhang SH, He HW, Zhang CX, Yu DK, Shao RG. Cancer Gene Ther. 2013 Nov;20(11):622-9.
Proteomic analysis of wild-type and mutant huntingtin-associated proteins in - Proteomic analysis of wild-type and mutant huntingtin-associated proteins in
Culver BP, Savas JN, Park SK, Choi JH, Zheng S, Zeitlin SO, Yates JR 3rd, Tanese N. J Biol Chem. 2012 Jun 22;287(26):21599-614.