Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121311 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-GAB3 antibody (ab121311) at 1/250 dilutionLane 1 : RT4 cell lysateLane 2 : U-251 MG cell lysateLane 3 : Human Plasma Lane 4 : Human Liver lysateLane 5 : Human Tonsil lysatedeveloped using the ECL technique
ab121311 at 1/400 dilution staining GAB3 in Paraffin-embedded Human liver tissue by Immunohistochemistry.