Anti-GABA A Receptor alpha 2 antibody [N399/19]

• Supplier: Abcam
• Catalog: ab193311
• Prices: $359.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: N399/19
• Applications: IHC-P ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Fusion protein corresponding to Rat GABA A Receptor alpha 2 aa 350-450
• Description: Mouse Monoclonal
• Gene: GABRA2
• Conjugate: Unconjugated

Product images

All lanes : Anti-GABA A Receptor alpha 2 antibody [N399/19] (ab193311) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 4 : Pancreas (Rat) Tissue LysateLane 5 : Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816)Lane 6 : Pancreas (Mouse) Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab193311 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab193311 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

IHC image of GABA A Receptor alpha 2 staining in Human Normal Cerebral Cortex formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab193311, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre