Anti-GABA A Receptor alpha 5 antibody
| Name | Anti-GABA A Receptor alpha 5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10098 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog, Human |
| Antigen | Synthetic peptide corresponding to the middle region of Mouse GABA A Receptor alpha 5 (NP_795916 |
| Blocking Peptide | GABA A Receptor alpha 5 peptide |
| Description | Rabbit Polyclonal |
| Gene | GABRA5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-GABA A Receptor alpha 5 antibody (ab10098) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse brain lysateLane 3 : Mouse brain lysateLane 4 : Mouse brain lysateLysates/proteins at 40 µg per lane.SecondaryIRDye® 680LT-conjugated donkey anti-rabbit IgG polyclonal (undiluted)Performed under reducing conditions.
ICC/IF image of ab10098 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10098, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab10098 staining GABA A Receptor alpha 5 in rat hippocampus sections by Immunohistochemistry (Frozen sections).Rats were transcardially perfused with 4% PFA, the brain was cryoprotected in 30% sucrose before flash freezing. Coronal sections were taken using cryostat (20 micron sections) and mounted onto slides. Tissue was fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were then blocked using 10% serum for 1 hour at 20°C and then incubated with ab10098 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at a 1/600 dilution.An antigen retrieval step improved results (5% SDS for 5 minutes before standard IHC protocol).See Abreview
Anti-GABA A Receptor alpha 5 antibody (ab10098) at 1 µg/ml + Mouse Liver Lysate
Product References
Expression of GABAA alpha2-, beta1- and epsilon-receptors are altered - Expression of GABAA alpha2-, beta1- and epsilon-receptors are altered
Fatemi SH, Folsom TD, Rooney RJ, Thuras PD. Transl Psychiatry. 2013 Sep 10;3:e303.
Synaptic and extrasynaptic origin of the excitation/inhibition imbalance in the - Synaptic and extrasynaptic origin of the excitation/inhibition imbalance in the
Farisello P, Boido D, Nieus T, Medrihan L, Cesca F, Valtorta F, Baldelli P, Benfenati F. Cereb Cortex. 2013 Mar;23(3):581-93.
mRNA and protein levels for GABAAalpha4, alpha5, beta1 and GABABR1 receptors are - mRNA and protein levels for GABAAalpha4, alpha5, beta1 and GABABR1 receptors are
Fatemi SH, Reutiman TJ, Folsom TD, Rooney RJ, Patel DH, Thuras PD. J Autism Dev Disord. 2010 Jun;40(6):743-50.