Anti-GABA A Receptor beta 3 antibody [S87-25]
| Name | Anti-GABA A Receptor beta 3 antibody [S87-25] |
|---|---|
| Supplier | Abcam |
| Catalog | ab98968 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | S87-25 |
| Applications | IHC-F ICC/IF ICC/IF FC IHC-P WB |
| Species Reactivities | Mouse, Rat, Human, Bovine, Orangutan |
| Antigen | Fusion protein: APMDVHNEMN EVAGSVGDTR NSAISFDNSG IQYRKQSMPK EGHGRYMGDR SIPHKKTHLR RRSS, corresponding to amino acids 370-433 of Mouse GABA A Receptor beta 3 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | GABRB3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab98968 staining GABA A Receptor beta 3 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
![Anti-GABA A Receptor beta 3 antibody [S87-25] (ab98968) at 1/1000 dilution + Rat brain membrane lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/21835_ab98968-199520-ab98968wb.jpg)
Anti-GABA A Receptor beta 3 antibody [S87-25] (ab98968) at 1/1000 dilution + Rat brain membrane lysate
![Overlay histogram showing SH-SY5Y cells stained with ab98968 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98968, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/21836_GABA-A-Receptor-beta-3-Primary-antibodies-ab98968-1.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab98968 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98968, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.