Anti-GABA B Receptor 2 antibody [EP2411Y]
| Name | Anti-GABA B Receptor 2 antibody [EP2411Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab75838 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP2411Y |
| Applications | IHC-P WB ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues near the C terminus of human GABA B Receptor 2 |
| Description | Rabbit Monoclonal |
| Gene | GABBR2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab75838, at a 1/100 dilution, staining GABA B Receptor 2 in paraffin embedded human brain tissue by Immunohistochemistry.
![Anti-GABA B Receptor 2 antibody [EP2411Y] (ab75838) at 1/500 dilution + human cerebellum lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/21883_ab75838wb.gif)
Anti-GABA B Receptor 2 antibody [EP2411Y] (ab75838) at 1/500 dilution + human cerebellum lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescence analysis of GABA B Receptor 2 using ab75838 at 1/400 dilution. Left panel = normal rat DRG neurons. Right panel = rat DRG neurons transfected with siRNAs to disrupt GABA B Receptor 2.
Overlay histogram showing SH-SY5Y cells stained with ab75838 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75838, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Differential modulation of nicotine-induced gemcitabine resistance by GABA - Differential modulation of nicotine-induced gemcitabine resistance by GABA
Banerjee J, Al-Wadei HA, Al-Wadei MH, Dagnon K, Schuller HM. BMC Cancer. 2014 Sep 27;14:725.
gamma-Aminobutyric acid type B (GABAB) receptor expression is needed for - gamma-Aminobutyric acid type B (GABAB) receptor expression is needed for
Cuny H, de Faoite A, Huynh TG, Yasuda T, Berecki G, Adams DJ. J Biol Chem. 2012 Jul 6;287(28):23948-57.