Anti-GABA Receptor Epsilon antibody
| Name | Anti-GABA Receptor Epsilon antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab35971 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human, Rat |
| Antigen | A region within synthetic peptide: KNSWKLFQFD FTGVSNKTEI ITTPVGDFMV MTIFFNVSRR FGYVAFQNYV, corresponding to intracellular amino acids 237-286 of Human GABA Receptor Epsilon Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | GABRE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab35971 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35971, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab35971 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab35971, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of male, virgin female, G17 pregnant, and 30 d post-partum rat medullary neurons, staining GABA Receptor Epsilon with ab35971 at 1/1000 dilution.
Product References
Expression of GABAA alpha2-, beta1- and epsilon-receptors are altered - Expression of GABAA alpha2-, beta1- and epsilon-receptors are altered
Fatemi SH, Folsom TD, Rooney RJ, Thuras PD. Transl Psychiatry. 2013 Sep 10;3:e303.
Increased GABA(A) receptor epsilon-subunit expression on ventral respiratory - Increased GABA(A) receptor epsilon-subunit expression on ventral respiratory
Hengen KB, Nelson NR, Stang KM, Johnson SM, Crader SM, Watters JJ, Mitchell GS, Behan M. PLoS One. 2012;7(1):e30608.
Changes in ventral respiratory column GABAaR epsilon- and delta-subunits during - Changes in ventral respiratory column GABAaR epsilon- and delta-subunits during
Hengen KB, Gomez TM, Stang KM, Johnson SM, Behan M. Am J Physiol Regul Integr Comp Physiol. 2011 Feb;300(2):R272-83. doi: