All lanes : Anti-Galactosidase alpha antibody [EP5828(2)] (ab168341) at 1/1000 dilutionLane 1 : MCF-7 cell lysatesLane 2 : 293T cell lysatesLane 3 : A431 cell lysatesLane 4 : HeLa cell lysatesLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human urinary bladder carcinoma tissue labeling Galactosidase alpha with ab168341 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling Galactosidase alpha with ab168341 at 1/50 dilution.
Immunofluorescence analysis of HeLa cells labeling Galactosidase alpha with ab168341 at 1/50 dilution.
Western blot analysis labeling Galactosidase alpha on immunoprecipitation pellet from MCF-7 cell lysate using ab168341 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.