Anti-Galectin 1 antibody
| Name | Anti-Galectin 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab25138 |
| Prices | $401.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF FC ELISA WB |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Rabbit Polyclonal |
| Gene | LGALS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab25138 staining Galectin 1 in Human umbilical cord blood mescenchymal stem cells by Flow Cytometry. Cells were fixed with formaldehyde. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 30 minutes at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polycloanl (1/2000) was used as the secondary antibody.Purple - isotype control, Green - Galectin 1.See Abreview
ICC/IF image of ab25138 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25138, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab25138 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25138, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Galectin 1 antibody (ab25138) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
A short treatment with galactomannan GM-CT-01 corrects the functions of freshly - A short treatment with galactomannan GM-CT-01 corrects the functions of freshly
Demotte N, Bigirimana R, Wieers G, Stroobant V, Squifflet JL, Carrasco J, Thielemans K, Baurain JF, Van Der Smissen P, Courtoy PJ, van der Bruggen P. Clin Cancer Res. 2014 Apr 1;20(7):1823-33.
A galectin-3 ligand corrects the impaired function of human CD4 and CD8 - A galectin-3 ligand corrects the impaired function of human CD4 and CD8
Demotte N, Wieers G, Van Der Smissen P, Moser M, Schmidt C, Thielemans K, Squifflet JL, Weynand B, Carrasco J, Lurquin C, Courtoy PJ, van der Bruggen P. Cancer Res. 2010 Oct 1;70(19):7476-88.
Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A - Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A
Boraldi F, Annovi G, Carraro F, Naldini A, Tiozzo R, Sommer P, Quaglino D. Biochim Biophys Acta. 2007 Nov;1774(11):1402-13. Epub 2007 Aug 22.