Anti-Galectin 1 antibody

• Supplier: Abcam
• Catalog: ab58085
• Prices: $378.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Applications: WB IHC-P ICC/IF ICC/IF ELISA
• Species Reactivities: Human
• Antigen: Recombinant full length protein (GST-tag) corresponding to Human Galectin 1 aa 1-135
• Description: Mouse Monoclonal
• Gene: LGALS1
• Conjugate: Unconjugated

Product images

Immunofluorescence analysis of HeLa cells labeling Galectin 1 with ab58085 at 10 ug/ml.

ab58085 (1µg/ml) staining galectin in human heart (left ventricle), using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of cardiomyocytes.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Detection limit for recombinant GST tagged Galectin 1 is 1 ng/ml as a capture antibody.

Product References

Modulation of ionotropic glutamate receptor function by vertebrate galectins. - Modulation of ionotropic glutamate receptor function by vertebrate galectins.

Copits BA, Vernon CG, Sakai R, Swanson GT. J Physiol. 2014 May 15;592(Pt 10):2079-96.

Ligand-induced Flt3-downregulation modulates cell death associated proteins and - Ligand-induced Flt3-downregulation modulates cell death associated proteins and

Oveland E, Gjertsen BT, Wergeland L, Selheim F, Fladmark KE, Hovland R. Leuk Res. 2009 Feb;33(2):276-87.