Anti-Galectin 7 antibody
| Name | Anti-Galectin 7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10482 |
| Prices | $395.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human, Chimpanzee, Ape, Orangutan |
| Antigen | Immunogen was a synthetic peptide, which represented a portion of human Lectin, Galactoside-binding 7 encoded within exon 4 (LocusLink ID 3963) |
| Description | Rabbit Polyclonal |
| Gene | LGALS7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab10482 staining Galectin7 in Human cervical tumor and immortalized keratinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS and blocked with 1% BSA for 1 hourat 25°C. Samples were incubated with primary antibody (1/1000 in 1% BSA/PBS) for 24 hours at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody. SiHa cells (negative control - very low Gal-7 mRNA) Immortilized keratinocytes (positive control - high Gal-7 mRNA)
IHC image of ab10482 staining in human Hodgkin's lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10482, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Novel potential interacting partners of fibronectin in spontaneous animal model - Novel potential interacting partners of fibronectin in spontaneous animal model
Treutlein G, Dorsch R, Euler KN, Hauck SM, Amann B, Hartmann K, Deeg CA. PLoS One. 2012;7(12):e51391.
Identification of differentially expressed proteins in spontaneous thymic - Identification of differentially expressed proteins in spontaneous thymic
Honore B, Buus S, Claesson MH. Proteome Sci. 2008 Jun 10;6:18.