Anti-galectin 9 antibody
| Name | Anti-galectin 9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab69630 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human galectin 9 aa 51-100 (internal sequence) |
| Description | Rabbit Polyclonal |
| Gene | LGALS9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-galectin 9 antibody (ab69630) at 1/500 dilutionLane 1 : Extracts from HepG2 cellsLane 2 : Extracts from HepG2 cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
ab69360 (4µg/ml) staining Galectin 9 in human lymphoma using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and secreted staining throughout the tumour.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
Product References
Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis - Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis
Jiang J, Jin MS, Kong F, Cao D, Ma HX, Jia Z, Wang YP, Suo J, Cao X. PLoS One. 2013 Dec 10;8(12):e81799.
Galectin-9 protein expression in endothelial cells is positively regulated by - Galectin-9 protein expression in endothelial cells is positively regulated by
Alam S, Li H, Margariti A, Martin D, Zampetaki A, Habi O, Cockerill G, Hu Y, Xu Q, Zeng L. J Biol Chem. 2011 Dec 23;286(51):44211-7.