IHC image of galectin 9 staining in Human normal Tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab153673, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![All lanes : Anti-galectin 9 antibody [1G3] (ab153673) at 5 µg/mlLane 1 : HeLa transfected with Galectin 9 whole cell lysateLane 2 : HeLa (wild type control) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-mouse IgG polyclonal antibody (HRP conjugated) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Additional bands at : 18 kDa,35 kDa. We are unsure as to the identity of these extra bands.Exposure time : 30 seconds](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22298_ab153673-245166-ab153673dsimage.jpg)
All lanes : Anti-galectin 9 antibody [1G3] (ab153673) at 5 µg/mlLane 1 : HeLa transfected with Galectin 9 whole cell lysateLane 2 : HeLa (wild type control) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-mouse IgG polyclonal antibody (HRP conjugated) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Additional bands at : 18 kDa,35 kDa. We are unsure as to the identity of these extra bands.Exposure time : 30 seconds