All lanes : Anti-GALR3 antibody [EPR15055] - C-terminal (ab190694) at 1/2000 dilutionLane 1 : Human cerebellum tissue lysateLane 2 : Human hippocampus tissue lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-GALR3 antibody [EPR15055] - C-terminal (ab190694) at 1/2000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLane 3 : C6 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of Human cerebellum tissue lysate labeling GALR3 with ab190694 at 1/50 dilution (lane 1) and a PBS control (lane 2). Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.