All lanes : Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : MDA-MB-435 cell lysateLane 3 : Human fetal brain lysateLane 4 : Human fetal liver lysateLane 5 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Immunofluorescent analysis of HepG2 cells labeling GALT with ab178406 at 1/50 dilution. DAPI nuclear staining (blue).
Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing GALT, immunoprecipitated using ab178406 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.