All lanes : Anti-gamma Actin antibody (ab194952) at 1/1000 dilutionLane 1 : K562 cell line lysateLane 2 : HepG2 cell line lysateLysates/proteins at 35 µg per lane.
All lanes : Anti-gamma Actin antibody (ab194952) at 1/1000 dilutionLane 1 : 293 cell lysate non-transfectedLane 2 : 293 cell lysate transiently transfected with gamma ActinLysates/proteins at 2 µg per lane.
Flow cytometry of K562 cells using ab194952 at 1/10 (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of HepG2 cells labeling gamma Actin with ab194952 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling gamma Actin with ab194952 at 1/100.