Anti-GAPDH antibody
| Name | Anti-GAPDH antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22555 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human, Monkey, Sheep, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog, Turkey, Pig, Duck |
| Antigen | Synthetic peptide: DAPMFVMGVNHFKYA , corresponding to amino acids 126-140 of Human GAPDH |
| Description | Rabbit Polyclonal |
| Gene | GAPDH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-GAPDH antibody (ab22555) at 0.1 µg/ml + Rat heart protein extractPerformed under reducing conditions.Observed band size : 36 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-GAPDH antibody (ab22555) at 1/5000 dilutionLane 1 : Mouse Tissue Lysate- Whole brainLane 2 : Mouse Tissue Lysate- Whole brainLane 3 : Mouse Tissue Lysate- Whole brainLane 4 : Mouse Tissue Lysate- Whole brainLane 5 : Mouse Tissue Lysate- Whole brainLane 6 : Mouse Tissue Lysate- Whole brainLane 7 : Mouse Tissue Lysate- Whole brainLane 8 : Mouse Tissue Lysate- Whole brainLysates/proteins at 50 µg per lane.This image is courtesy of an anonymous AbreviewAll Lanes: ab22555 (1/15000) staining GAPDH in Mouse brain whole tissue lysate (50 µg). Blocking Step: 5% Milk for 1 hour at 20°C. Secondary Antibody: HRP-conjugated Goat anti-rabbit IgG polyclonal (1/30000).See Abreview
ab22555 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab22555 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Matrix metalloproteinase-9 is required for tumor vasculogenesis but not for - Matrix metalloproteinase-9 is required for tumor vasculogenesis but not for
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