Anti-GAPDH antibody [6C5]
| Name | Anti-GAPDH antibody [6C5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab8245 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 6C5 |
| Applications | ELISA ICC/IF WB ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Rabbit, Chicken, Hamster, Cat, Dog, Human, Pig, Xenopus, Drosophila, Fish, Monkey, Zebrafish, Monkey, Monkey, Horse, Guinea Pig, Xenopus |
| Antigen | Rabbit muscle GAPDH |
| Description | Mouse Monoclonal |
| Gene | GAPDH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab8245 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab8245 at 5μg/ml and ab6046 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-Mouse AlexaFluor® 488 (ab150117) at 2 μg/ml (shown in green) and anti-Rabbit AlexaFluor® 594 (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
![All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 20000 Lane 1 : Mouse hippocampus whole cell lysateLane 2 : Rat hippocampus whole cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated Rabbit anti-mouse at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22765_ab8245-209020-ab8245wb.jpg)
All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 20000 Lane 1 : Mouse hippocampus whole cell lysateLane 2 : Rat hippocampus whole cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated Rabbit anti-mouse at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 2.5 µg/mlLane 1 : HeLa NuclearLane 2 : HeLa whole cell lysateLane 3 : A431 cell lysateLane 4 : Jurkat cell lysateLane 5 : HEK293 cell lysateLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor anti-mouse at 1/5000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22766_ab8245_2.jpg)
All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 2.5 µg/mlLane 1 : HeLa NuclearLane 2 : HeLa whole cell lysateLane 3 : A431 cell lysateLane 4 : Jurkat cell lysateLane 5 : HEK293 cell lysateLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor anti-mouse at 1/5000 dilutionPerformed under reducing conditions.
![All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 1/20000 dilutionLane 1 : Rabbit aorta, whole tissue lysate at 10 µgLane 2 : BlankLane 3 : Mouse aorta, whole tissue lysate at 5 µgLane 4 : Mouse aorta, whole tissue lysate at 7.5 µgLane 5 : Mouse aorta, whole tissue lysate at 10 µgLane 6 : Mouse aorta, whole tissue lysate at 12.5 µgLane 7 : Mouse aorta, whole tissue lysate at 15 µgSecondaryHRP conjugated sheep anti-mouse antibody at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22767_ab8245_4.jpg)
All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 1/20000 dilutionLane 1 : Rabbit aorta, whole tissue lysate at 10 µgLane 2 : BlankLane 3 : Mouse aorta, whole tissue lysate at 5 µgLane 4 : Mouse aorta, whole tissue lysate at 7.5 µgLane 5 : Mouse aorta, whole tissue lysate at 10 µgLane 6 : Mouse aorta, whole tissue lysate at 12.5 µgLane 7 : Mouse aorta, whole tissue lysate at 15 µgSecondaryHRP conjugated sheep anti-mouse antibody at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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HER2/ErbB2 activates HSF1 and thereby controls HSP90 clients including MIF in - HER2/ErbB2 activates HSF1 and thereby controls HSP90 clients including MIF in
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