Anti-GART antibody [EPR11622]

• Supplier: Abcam
• Catalog: ab169550
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR11622
• Applications: ICC/IF ICC/IF WB IHC-P IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment corresponding to residues in Human GART (UniProt P22102)
• Description: Rabbit Monoclonal
• Gene: GART
• Conjugate: Unconjugated

Product images

All lanes : Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : K562 cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling GART with ab169550 at 1/50 dilution.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GART with ab169550 at 1/50 dilution.

ab169550 staining GART in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.See Abreview

Immunofluorescent analysis of HepG2 cells labeling GART with ab169550 at 1/100 dilution.

Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysateSecondaryHRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG