ab75044, at a 1/50 dilution, staining Gastric Mucin in formalin fixed, paraffin embedded human stomach tissue by Immunohistochemistry. Peroxidase conjugate and AEC chromogen were used. Note cytoplasmic staining of mucous secreting cells.
ab75044 staining Gastric Mucin in human stomach, small intestine and colon cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0, 100°C for 20 minutes. Samples were then incubated with the primary antibody for 20 minutes at 25°C. A HRP-conjugated goat anti-mouse/rabbit polyclonal was used undiluted as the secondary antibody.See Abreview