Anti-GATA3 (phospho S308) antibody [EPR18118]

• Supplier: Abcam
• Catalog: ab186371
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR18118
• Applications: WB IHC-P DB IP
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GATA3 aa 300-400 (phospho S308)
• Description: Rabbit Monoclonal
• Gene: GATA3
• Conjugate: Unconjugated

Product images

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on trophoblast cells of Human placenta is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

All lanes : Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371) at 1/10000 dilutionLane 1 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLane 2 : Jurkat whole cell lysates treated with 1mM 8-Bromo-cAMP for 6 hoursLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

GATA3 (phospho S308) was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with 1mM 8-Bromo-cAMP for 6 hours with ab186371 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab186371 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as secondary antibody at 1/1500 dilution.Lane 1: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours, 10 µg (Input).Lane 2: ab186371 IP in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab186371 in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds.

Dot blot analysis of GATA3 (phospho S308) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab186371 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST..Exposure time: 3 minutes.