All lanes : Anti-GBE1 antibody [1D11] (ab124023) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY GBE1 Lysates/proteins at 5 µg per lane.
All lanes : Anti-GBE1 antibody [1D11] (ab124023) at 1/2000 dilutionLane 1 : HepG2 cell line lysateLane 2 : HeLa cell line lysateLane 3 : HT29 cell line lysateLane 4 : A549 cell line lysateLane 5 : COS7 cell line lysateLane 6 : Jurkat cell line lysateLane 7 : MDCK cell line lysateLane 8 : PC12 cell line lysateLane 9 : MCF7 cell line lysateLysates/proteins at 35 µg per lane.
ab124023, at 1/150, staining GBE1 in paraffin embedded Human colon tissue by Immunohistochemistry.
ab124023, at 1/150, staining GBE1 in paraffin embedded Human ovary tissue by Immunohistochemistry.
ab124023, at 1/150, staining GBE1 in paraffin embedded Human endometrium tissue by Immunohistochemistry.
ab124023, at 1/100, immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.
ab124023, at 1/100, immunofluorescent staining of HepG2 cells.
HEK293T cells transfected with either pCMV6-ENTRY GBE1 (Red) or empty vector control plasmid (Blue) were immunostained with ab124023, at 1/1000, and then analyzed by Flow cytometry.
Flow cytometric analysis of Jurkat cells, using ab124023, at 1/100, (Red) compared to a nonspecific negative control antibody (Blue).