All lanes : Anti-GCLC antibody [EP13475] (ab190685) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : A431 cell lysateLane 3 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-GCLC antibody [EP13475] (ab190685) at 1/50000 dilution + HepG2 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-GCLC antibody [EP13475] (ab190685) at 1/10000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation of A549 cell lysates using ab190685. Detection of GCLC utilized ab190685 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated seondary antibody at 1/1000 dilution.