Anti-GCNF antibody [4G8] (ab135241) at 1/500 dilution + Human GCNF recombinant protein
All lanes : Anti-GCNF antibody [4G8] (ab135241) at 1/500 dilutionLane 1 : HEK293 cell lysates, non-transfectedLane 2 : HEK293 cell lysates, transfected with Human GCNF recombinant fragment (amino acids 65-118)-hIgGFc
All lanes : Anti-GCNF antibody [4G8] (ab135241) at 1/500 dilutionLane 1 : K562 cell lysateLane 2 : NTERA 2 cell lysateLane 3 : HEK293 cell lysateLane 4 : HUVE 12 cell lysateLane 5 : HeLa cell lysate
Flow cytometric analysis of K562 cells labelling GCNF with ab135241 at 1/200 dilution (green). Negative control (red).
Immunofluorescent analysis of HepG2 cells labelling GCNF with ab135241 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemical analysis of Paraffin-embedded Human cerebellum tissue labelling GCNF with ab135241 at 1/200 dilution followed by DAB staining.
Immunohistochemical analysis of Paraffin-embedded Human rectum cancer tissue labelling GCNF with ab135241 at 1/200 dilution followed by DAB staining.