ab19479 staining the GCSF Receptor (red) in Mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue were fixed with paraformaldehyde and blocked with 5% BSA for 1 hoaur at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 12 hours at 4°C. A Cy3®-conjugated Donkey anti-mouse polyclonal (1/500) was used as secondary antibody. DAPI staining in blue.See Abreview
![Overlay histogram showing U937 cells stained with ab19479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19479, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/23620_GCSF-Receptor-Primary-antibodies-ab19479-3.jpg)
Overlay histogram showing U937 cells stained with ab19479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19479, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.