Anti-GCSH antibody [EPR14567(B)] - C-terminal

• Supplier: Abcam
• Catalog: ab192613
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14567(B)
• Applications: WB IHC-P FC IP ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GCSH aa 150 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: GCSH
• Conjugate: Unconjugated

Product images

Western blot analysis of immunoprecipitation pellet from Human fetal kidney lysate immunoprecipitated using ab192613 at 1/100 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling GCSH with ab192613 at 1/360 dilution (red) or a rabbit IgG (negative) (blue), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling GCSH with ab192613 at 1/500 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling GCSH with ab192613 at 1/8000 dilution dilution (0.25 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling GCSH with ab192613 at 1/8000 dilution dilution (0.25 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GCSH with ab192613 at 1/8000 dilution dilution (0.25 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

All lanes : Anti-GCSH antibody [EPR14567(B)] - C-terminal (ab192613) at 1/10000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal liver lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-GCSH antibody [EPR14567(B)] - C-terminal (ab192613) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : T47D cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution