All lanes : Anti-GDF15 antibody [23B3D2.H5] (ab106111) at 1/1000 dilutionLane 1 : Pichia pastoris (yeast) whole cell lysate expressing GDF15 H variant with SUMO expression tag Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing GDF15 D variant with SUMO expression tag Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing GDF15 H variantLane 4 : Pichia pastoris (yeast) whole cell lysate expressing GDF15 D variantSecondaryPeroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilutionPerformed under reducing conditions.