Anti-GDF15 antibody [7C12.B3.F2]

• Supplier: Abcam
• Catalog: ab106112
• Prices: $378.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: unknown
• Clone: 7C12.B3.F2
• Applications: ELISA WB ICC/IF ICC/IF
• Species Reactivities: Human, Chimpanzee, Monkey
• Antigen: A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein
• Description: Mouse Monoclonal
• Gene: GDF15
• Conjugate: Unconjugated

Product images

ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.

All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilutionLane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tagLane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variantSecondaryPeroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilutionPerformed under reducing conditions.