All lanes : Anti-GEF H1 antibody (ab155785) at 1/2000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLysates/proteins at 30 µg per lane.
All lanes : Anti-GEF H1 antibody (ab155785) at 1/1000 dilutionLane 1 : hMSC-3A6 whole cell lysate, mock transfectedLane 2 : hMSC-3A6 whole cell lysate, transfected with GEF H1 siRNALysates/proteins at 10 µg per lane.
All lanes : Anti-GEF H1 antibody (ab155785) at 1/1000 dilutionLane 1 : RAW264.7 whole cell lysateLane 2 : C2C12 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of methanol-fixed (-20°C 100% MeOH for 5 min) HeLa cells, labeling GEF H1 with ab155785 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue, labeling GEF H1 with ab155785 at 1/500 dilution.