Anti-Gelsolin antibody
| Name | Anti-Gelsolin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab74420 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human Gelsolin |
| Description | Rabbit Polyclonal |
| Gene | GSN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Gelsolin antibody (ab74420) at 1 µg/mlLane 1 : Kidney (Rat) Tissue Lysate Lane 2 : Spleen (Mouse) Tissue Lysate Lane 3 : Thymus (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab74420 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74420, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 1µg/ml.
ab74420 staining Gelsolin in human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).The black arrow indicates a gelsolin-positive Lewy body.
Product References
Chitinase-like proteins are candidate biomarkers for sepsis-induced acute kidney - Chitinase-like proteins are candidate biomarkers for sepsis-induced acute kidney
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Quantitative protein and mRNA profiling shows selective post-transcriptional - Quantitative protein and mRNA profiling shows selective post-transcriptional
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Neutrophils require SHP1 to regulate IL-1beta production and prevent inflammatory - Neutrophils require SHP1 to regulate IL-1beta production and prevent inflammatory
Croker BA, Lewis RS, Babon JJ, Mintern JD, Jenne DE, Metcalf D, Zhang JG, Cengia LH, O'Donnell JA, Roberts AW. J Immunol. 2011 Jan 15;186(2):1131-9.
Gelsolin co-occurs with Lewy bodies in vivo and accelerates alpha-synuclein - Gelsolin co-occurs with Lewy bodies in vivo and accelerates alpha-synuclein
Welander H, Bontha SV, Nasstrom T, Karlsson M, Nikolajeff F, Danzer K, Kostka M, Kalimo H, Lannfelt L, Ingelsson M, Bergstrom J. Biochem Biophys Res Commun. 2011 Aug 19;412(1):32-8. doi:
Cytochrome P450 2B1 mediates complement-dependent sublytic injury in a model of - Cytochrome P450 2B1 mediates complement-dependent sublytic injury in a model of
Liu H, Tian N, Arany I, Bigler SA, Waxman DJ, Shah SV, Baliga R. J Biol Chem. 2010 Dec 24;285(52):40901-10.