Anti-Gelsolin antibody [EPR1942]

• Supplier: Abcam
• Catalog: ab109014
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1942
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)
• Description: Rabbit Monoclonal
• Gene: GSN
• Conjugate: Unconjugated

Product images

Overlay histogram showing HL60 cells stained with ab109014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109014, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/50000 dilutionLane 1 : MCF7 cell lysateLane 2 : HL60 cell lysateLane 3 : A431 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human kidney tissue using ab109014 at 1/250 dilution.

Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using ab109014 at 1/250 dilution.

Immunofluorescent staining of Gelsolin in HeLa cells using ab109014 at 1/100 dilution.