Anti-Gephyrin antibody

• Supplier: Abcam
• Catalog: ab32206
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-F WB
• Species Reactivities: Mouse, Rat, Chicken, Human, Xenopus
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse Gephyrin
• Blocking Peptide: Mouse Gephyrin peptide
• Description: Rabbit Polyclonal
• Gene: GPHN
• Conjugate: Unconjugated

Product images

All lanes : Anti-Gephyrin antibody (ab32206) at 1 µg/mlLane 1 : Brain (Mouse) Whole Cell Lysate - AdultLane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µgLane 3 : Brain (Mouse) Whole Cell Lysate - Adult at 20 µg with Mouse Gephyrin peptide (ab32205) at 1 µg/mlLane 4 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µg with Mouse Gephyrin peptide (ab32205) at 1 µg/mlSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Immunofluorescent staining for Gephyrin in rat brain cortex using Rabbit polyclonal to Gephyrin (ab32206; 1/1000). The staining is located in the cytoplasm and in some of the processes of cortical neurons. This staining is observed in many other brain areas. The picture was acquired using a X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab32206 was incubated overnight at 1/1000 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.

All lanes : Anti-Gephyrin antibody (ab32206) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue Lane 4 : Heart (Mouse) Tissue LysateLane 5 : Kidney (Mouse) Tissue LysateLane 6 : Pancreas (Mouse) Tissue Lysate (ab29363)Lane 7 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 9 : Liver (Rat) Tissue Lysate Lane 10 : Heart (Rat) Tissue Lysate Lane 11 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab32206 stained MEF1 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab32206, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

ICC/IF image of ab32206 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32206, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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Nunez-Parra A, Maurer RK, Krahe K, Smith RS, Araneda RC. Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):14777-82. doi:

Dynamic changes in neurexins' alternative splicing: role of Rho-associated - Dynamic changes in neurexins' alternative splicing: role of Rho-associated

Rozic G, Lupowitz Z, Piontkewitz Y, Zisapel N. PLoS One. 2011 Apr 12;6(4):e18579.

Morphological changes and synaptogenesis of corticothalamic neurons in the - Morphological changes and synaptogenesis of corticothalamic neurons in the

Hsu CI, Ho TS, Liou YR, Chang YC. Cereb Cortex. 2011 Apr;21(4):884-95.

Early developmental alterations in GABAergic protein expression in fragile X - Early developmental alterations in GABAergic protein expression in fragile X

Adusei DC, Pacey LK, Chen D, Hampson DR. Neuropharmacology. 2010 Sep;59(3):167-71.

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Gawlak M, Gorkiewicz T, Gorlewicz A, Konopacki FA, Kaczmarek L, Wilczynski GM. Neuroscience. 2009 Jan 12;158(1):167-76.

Fluctuations in brain concentrations of neurosteroids are not associated to - Fluctuations in brain concentrations of neurosteroids are not associated to

Sassoe-Pognetto M, Follesa P, Panzanelli P, Perazzini AZ, Porcu P, Sogliano C, Cherchi C, Concas A. Brain Res. 2007 Sep 12;1169:1-8. Epub 2007 Jul 13.