All lanes : Anti-GGA3 antibody [EPR14340] (ab180951) at 1/5000 dilutionLane 1 : SH-SY5Y cell line with NFDM/TBSTLane 2 : 293 cell line with NFDM/TBSTLane 3 : A431 cell line with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human Iifiltrating duct carcinoma of breast tissue labeling GGA3 using ab180951 at a 1/50 dilution, and counterstained with Hematoxylin. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling GGA3 using ab180951 at a 1/20 dilutioin, or Rabbit monoclonal IgG (isotype control). Goat anti rabbit IgG (FITC) at a 1/50 dilution was used as a secondary antibody.