Anti-GHRHR antibody
| Name | Anti-GHRHR antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28692 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: RTRAKWTTPSRSAAKVLTSM conjugated to KLH, corresponding to amino acids 403-422 of Human GHRHR Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | GHRHR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab28692 staining Human GHRHR in human ovarian carcinoma samples by immunohistochemistry using paraffin embedded tissue.
Anti-GHRHR antibody (ab28692) at 1/500 dilution + LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab28692 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28692, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone - Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone
Jaszberenyi M, Rick FG, Popovics P, Block NL, Zarandi M, Cai RZ, Vidaurre I, Szalontay L, Jayakumar AR, Schally AV. Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):781-6.
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GHRH antagonist MZ-5-156 increases the expression of AMPK in A549 lung cancer - GHRH antagonist MZ-5-156 increases the expression of AMPK in A549 lung cancer
Siejka A, Barabutis N, Schally AV. Cell Cycle. 2011 Nov 1;10(21):3714-8.
Antagonists of growth hormone-releasing hormone inhibit the proliferation of - Antagonists of growth hormone-releasing hormone inhibit the proliferation of
Siejka A, Schally AV, Block NL, Barabutis N. Prostate. 2010 Jul 1;70(10):1087-93.
Agonist of growth hormone-releasing hormone as a potential effector for survival - Agonist of growth hormone-releasing hormone as a potential effector for survival
Ludwig B, Ziegler CG, Schally AV, Richter C, Steffen A, Jabs N, Funk RH, Brendel MD, Block NL, Ehrhart-Bornstein M, Bornstein SR. Proc Natl Acad Sci U S A. 2010 Jul 13;107(28):12623-8. doi:
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Immunocytochemical localisation of plasma membrane GHRH receptors in human - Immunocytochemical localisation of plasma membrane GHRH receptors in human
Schulz S, Rocken C, Schulz S. Eur J Cancer. 2006 Sep;42(14):2390-6. Epub 2006 Aug 14.