Anti-GIPC1 antibody
| Name | Anti-GIPC1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5951 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide: GAIGDAKVGRY, corresponding to C terminal amino acids 323-333 of Human GIPC1 |
| Description | Goat Polyclonal |
| Gene | GIPC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)
ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.See Abreview
ICC/IF image of ab5951 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5951, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. - GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion.
Wu D, Haruta A, Wei Q. J Biol Chem. 2010 Sep 10;285(37):28643-50.
Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function - Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function
Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Konig I, Astanina E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G. PLoS Biol. 2009 Jan 27;7(1):e25.
Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the - Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the
Zerbinatti CV, Cordy JM, Chen CD, Guillily M, Suon S, Ray WJ, Seabrook GR, Abraham CR, Wolozin B. Mol Neurodegener. 2008 Mar 18;3:5.