All lanes : Anti-GIPC3 antibody [EPR15315] (ab186426) at 1/10000 dilutionLane 1 : K562 lysateLane 2 : Human lymph node lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/10000 dilution
Anti-GIPC3 antibody [EPR15315] (ab186426) at 1/50000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of GIPC3 in HeLa cell lysate immunoprecipitated using ab186426 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.