Anti-Gli1 antibody
| Name | Anti-Gli1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab49314 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Dog, Human, Rat, Rabbit, Horse, Guinea Pig, Bovine, Cat |
| Antigen | A region within Synthetic peptide: TNPSCGHPEV GRLGGGPALY PPPEGQVCNP LDSLDLDNTQ LDFVAILDEP , corresponding to amino acids 1007-1056 of Human Gli1 Run BLAST with Run BLAST with |
| Blocking Peptide | Human Gli1 peptide |
| Description | Rabbit Polyclonal |
| Gene | GLI1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
developed using the ECL techniquePerformed under reducing conditions.
Lane 1 : MARKERLane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/mlLane 1 : As aboveLane 2 : HepG2 cell lysate at 10 µgSecondaryLane 1 :Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Lane 1 : Anti-Gli1 antibody (ab49314) at 1.25 µg/mlLane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/mlLane 3 : Anti-Gli1 antibody (ab49314) at 5 µg/mlLane 1 : Cell lysate prepared from human HepG2 cellsLane 2 : Cell lysate prepared from human HepG2 cellsLane 3 : Cell lysate prepared from human HepG2 cellsLysates/proteins at 25 µg per lane.
ICC/IF image of ab49314 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49314, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
SOX2 regulates self-renewal and tumorigenicity of human melanoma-initiating - SOX2 regulates self-renewal and tumorigenicity of human melanoma-initiating
Santini R, Pietrobono S, Pandolfi S, Montagnani V, D'Amico M, Penachioni JY, Vinci MC, Borgognoni L, Stecca B. Oncogene. 2014 Sep 18;33(38):4697-708.
Blocking signaling at the level of GLI regulates downstream gene expression and - Blocking signaling at the level of GLI regulates downstream gene expression and
Shahi MH, Holt R, Rebhun RB. PLoS One. 2014 May 8;9(5):e96593.
Targeting Gli transcription activation by small molecule suppresses tumor growth. - Targeting Gli transcription activation by small molecule suppresses tumor growth.
Bosco-Clement G, Zhang F, Chen Z, Zhou HM, Li H, Mikami I, Hirata T, Yagui-Beltran A, Lui N, Do HT, Cheng T, Tseng HH, Choi H, Fang LT, Kim IJ, Yue D, Wang C, Zheng Q, Fujii N, Mann M, Jablons DM, He B. Oncogene. 2014 Apr 17;33(16):2087-97.
Modulation of lipid metabolic defects rescues cleft palate in Tgfbr2 mutant mice. - Modulation of lipid metabolic defects rescues cleft palate in Tgfbr2 mutant mice.
Iwata J, Suzuki A, Pelikan RC, Ho TV, Sanchez-Lara PA, Chai Y. Hum Mol Genet. 2014 Jan 1;23(1):182-93.
Megalin mediates the influence of sonic hedgehog on oligodendrocyte precursor - Megalin mediates the influence of sonic hedgehog on oligodendrocyte precursor
Ortega MC, Cases O, Merchan P, Kozyraki R, Clemente D, de Castro F. Glia. 2012 May;60(6):851-66.
WIP1 phosphatase modulates the Hedgehog signaling by enhancing GLI1 function. - WIP1 phosphatase modulates the Hedgehog signaling by enhancing GLI1 function.
Pandolfi S, Montagnani V, Penachioni JY, Vinci MC, Olivito B, Borgognoni L, Stecca B. Oncogene. 2013 Oct;32(40):4737-47.
Signaling through the primary cilium affects glial cell survival under a stressed - Signaling through the primary cilium affects glial cell survival under a stressed
Yoshimura K, Kawate T, Takeda S. Glia. 2011 Feb;59(2):333-44.
Up-regulation of sonic hedgehog contributes to TGF-beta1-induced epithelial to - Up-regulation of sonic hedgehog contributes to TGF-beta1-induced epithelial to
Maitah MY, Ali S, Ahmad A, Gadgeel S, Sarkar FH. PLoS One. 2011 Jan 13;6(1):e16068.
Inhibition of the sonic hedgehog pathway by cyplopamine reduces the CD133+/CD15+ - Inhibition of the sonic hedgehog pathway by cyplopamine reduces the CD133+/CD15+
Schiapparelli P, Shahi MH, Enguita-German M, Johnsen JI, Kogner P, Lazcoz P, Castresana JS. Cancer Lett. 2011 Nov 28;310(2):222-31.
In vitro and in vivo characterization of a novel Hedgehog signaling antagonist in - In vitro and in vivo characterization of a novel Hedgehog signaling antagonist in
Ferruzzi P, Mennillo F, De Rosa A, Giordano C, Rossi M, Benedetti G, Magrini R, Pericot Mohr Gl, Miragliotta V, Magnoni L, Mori E, Thomas R, Tunici P, Bakker A. Int J Cancer. 2012 Jul 15;131(2):E33-44.