All lanes : Anti-Gli1 antibody (ab151796) at 1/3000 dilutionLane 1 : NT2D1 whole cell lysateLane 2 : PC-3 whole cell lysateLane 3 : U87-MG whole cell lysateLane 4 : SK-N-SH whole cell lysateLysates/proteins at 30 µg per lane.
ab151796 staining Gli1 in rat bone tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 1% serum for 10 minutes at room temperature; antigen retrieval was enzymatic using trypsin. Samples were incubated with the undiluted primary antibody for 1 hour. An undiluted HRP-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Immunohistochemical analysis of paraffin-embedded Mouse Hippocampus tissue labeling Gli1 with ab151796 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labeling Gli1 with ab151796 at 1/500 dilution.