Anti-Gliomedin antibody
| Name | Anti-Gliomedin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab24483 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Rat Gliomedin |
| Description | Rabbit Polyclonal |
| Gene | GLDN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab24483 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24483, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Gliomedin antibody (ab24483) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Spinal Cord (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Product References
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Thaxton C, Pillai AM, Pribisko AL, Dupree JL, Bhat MA. Neuron. 2011 Jan 27;69(2):244-57.
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