All lanes : Anti-GLIPR1 antibody (ab138333) at 1 µg/mlLane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell LysateLane 2 : Lung (Human) Tissue LysateLane 3 : Lung (Human) Whole Cell Lysate - fetal normal tissueLane 4 : WI38 (Human lung fibroblast cell line) Whole Cell LysateLane 5 : Kidney (Human) Tissue Lysate - adult normal tissueLane 6 : Kidney (Human) Tissue Lysate - fetal normal tissueLane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab138333 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab138333, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.